You will learn how to recognise microbiological cultures. You will further get to know classical procedures for the selection and identification and how to analyse the microbiological concept. In addition you will get an insight into the needed media and in modern rapid methods.
Due to the limited number of participants and the supervision by experienced lab staff you will be able to do the work, to employ the methods and to make the analyses yourself. For that purpose there will be lab places with microscopes in the S2 lab for every participant. Accompanying presentations and a comprehensive documentation will provide the necessary knowledge base.
Background
Microbiological trainings are commonly based on presentations or theoretical workshops. Quite frequently there is either no advanced practical training at all or it is only very limited.
This completely new designed lab course is supposed to be an extension of the microbiological seminars as part of the GMP Certification Programme “Microbiological Laboratory Manager”.
Target Group
Personnel from labs for
Quality Control
Hygiene
Monitoring
with basic microbiological knowledge.
A special thanks for supporting this course goes to BioMérieux, Bruker, DoenitzProLab, Merck, Roche Diagnostics and Dastex GmbH.
Programme
Wednesday, 11 March 2020, 09.30-18.00 h
Welcome and organisational issues
Basic knowledge identification of microorganisms
Handling and examination of prepared cultures from different samples e.g. environmental monitoring, water on contact plates, swabs, membranes
Preparation of pure cultures
Introduction into the work with microscope and identification of microorganisms including staining procedures
Practice of biochemical rapid tests
Thursday, 12 March 2020, 08.30-18.00 h
Overview Molecular genetic methods and Mycoplasma detection
Identification with Maldi-TOF MS and FTIR spectroscopy
Practice in manual biochemical identification methods (Multi-Test-Systems, additional tests) and special media, e.g. chromogenic media using the prepared cultures
Practice in molecular genetic rapid methods using the example of Mycoplasma detection
Practice in identification of bacteria, yeasts and moulds by MALDI-TOF MS and FTIR spectroscopy
Friday, 13 March 2020, 08.00-15.30 h
Analysis of the results from molecular genetic rapid methods, MALDI-TOF MS, FTIR spectroscopy and manual biochemical methods
Increasing laboratory identification system performance with the use of quality control -Usage of reference strains
Consolidation and discussion of all results from bacterial identification
Basic knowledge and practice of yeast and moulds identification including microscopy
Beneficial literature and helpful addresses
This course is part of the GMP Certification Programme "ECA Certified Microbiological Laboratory Manager" Learn more
Testimonials